Stuart and Jerry were asked by Gru to watch the girls and keep them away from him.
Stuart, alongside Tom, are first shown cleaning up from Agnes' party but after seeing Gru be taken by Lucy, they Stuart pretending to be Superman chase after her. Stuart also notes that Silas Rams bottom has a name sounding like butt in the end, this leading Stuart and Tom to burst into laughter. Stuart is present along with Dave when Gru works undercover as the owner of a cupcake shop in The Paradise Shopping Mall. As a disguise he is dressed as a girl.